3.11. Cocoa Intake Alleviates Hepatic Oxidative Stress in Zucker Diabetic Fatty Rats
Cordero-Herrera, I.; Martín, M.A.; Goya, L.; Ramos, S. *
Background and objectives
Chronic hyperglycemia in diabetes is associated with oxidative
stress-mediated tissue damage. Cocoa and dark chocolate have demonstrated the ability to improve insulin sensitivity and modulate oxidative stress markers in diabetic patients. The present study is aimed at exploring the role of a cocoa-enriched diet in ameliorating the oxidative stress-induced damage in the liver of type 2 diabetic Zucker diabetic fatty (ZDF) rats.
Male ZDF rats were fed a control or cocoa-rich diet (10%), and Zucker lean (ZL) animals received the control diet. Hepatic reactive oxygen species (ROS), carbonyl and glutathione (GSH) levels, as well as superoxide dismutase (SOD), heme oxygenase (HO-1), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities, and nuclear factor erythroid-derived 2-like 2 (Nrf2), as well as p65-nuclear factor-kappaB (NF- B) expression were evaluated.
Cocoa diet reduced ROS levels and carbonyl content in the liver of ZDF animals. The diminished activity of SOD and the enhanced activity of HO-1 in ZDF-C were returned to ZL values upon cocoa administration. Cocoa did not restore the decreased GST activity in either ZDF groups in comparison to ZL rats. GSH content and activities of GPx, GR and CAT remained unaltered among all animal groups.
Moreover, the cocoa-rich diet suppressed total and phosphorylated Nrf2, as well as p65-NF- B-enhanced levels observed in ZDF rats.
Cocoa protects the hepatocytes by improving the antioxidant competence in the liver of young type 2 diabetic ZDF rats.
Grants AGL2010-17579, and CIBERDEM from MICINN. I. Cordero-Herrera is a fellow of the FPI predoctoral program (MICINN).
Referencias: ISCHOM II: Barcelona 2015. Nutrients